Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

Lihua Hou; Xiaoyang Li; Cong Wang; Xiaohong Cao; Haiyong Wang

doi:10.1007/s10295-013-1241-2

Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

J Ind Microbiol Biotechnol. 2013 Apr;40(3-4):393-7. doi: 10.1007/s10295-013-1241-2. Epub 2013 Feb 22.

Authors

Lihua Hou¹, Xiaoyang Li, Cong Wang, Xiaohong Cao, Haiyong Wang

Affiliation

¹ Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, People's Republic of China. lhhou@tju.edu.cn

PMID: 23430413
DOI: 10.1007/s10295-013-1241-2

Abstract

An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Deoxyribonucleases, Type II Site-Specific / genetics
Genes, Fungal
Plasmids / genetics*
Ploidies*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / genetics

Substances

Saccharomyces cerevisiae Proteins
HO protein, S cerevisiae
Deoxyribonucleases, Type II Site-Specific