DNA detection based on fluorogenic nanospheres

Xin Shu; Yonghui Liu; Jin Zhu

doi:10.1002/anie.201205628

DNA detection based on fluorogenic nanospheres

Angew Chem Int Ed Engl. 2012 Oct 29;51(44):11006-9. doi: 10.1002/anie.201205628. Epub 2012 Sep 28.

Authors

Xin Shu¹, Yonghui Liu, Jin Zhu

Affiliation

¹ Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093, China.

PMID: 23024037
DOI: 10.1002/anie.201205628

Abstract

High and dry fluorescence: Fluorogenic nanospheres (green, see scheme) are conjugated to DNA (pink) and used to detect target DNA (aqua). Upon addition of N-butylmorpholine (droplet), the nanospheres dissolve, releasing fluorophores, and intense blue fluorescence is emitted at the site of DNA hybridization. The separation of DNA hybridization and signal amplification gives high sensitivity (100 zmol) and selectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / analysis*
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemistry
Microscopy, Fluorescence
Nanospheres / analysis*
Nanospheres / chemistry

Substances

Fluorescent Dyes
DNA