Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China

Bioresour Technol. 2012 Oct:121:76-82. doi: 10.1016/j.biortech.2012.07.012. Epub 2012 Jul 16.

Abstract

Cultivation of Chlorella zofingiensis and nutrients removal in dairy wastewater were investigated in bench-scale outdoor ponds in winter, South China. The impacts of the two types of pH regulations, 5 ≈ 6% CO(2) and acetic acid (HAc) on this process were studied. After 6 days cultivation, the removal rates of total nitrogen (TN) and orthophosphate (PO(4)(3-)) using CO(2) regulation were better than those using HAc. The removal rates of PO(4)(3-) and TN were 97.5% and 51.7%, respectively using CO(2) regulation; 79.6% (TN) and 42.0% (PO(4)(3-)) were obtained using HAc regulation. Higher biomass, protein, sugar content, and stable pH control were found using CO(2) regulation. However, significantly higher lipid content (31.8%) was observed using HAc regulation. The dominant differences of fatty acids were the content of C18:1 and C18:3. The growth characteristics and environmental conditions especially during the typical logarithmic phase were also analyzed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / metabolism*
  • Amino Acids / analysis
  • Carbon Dioxide / metabolism*
  • Cell Culture Techniques*
  • China
  • Chlorella / chemistry
  • Chlorella / growth & development*
  • Dairying / methods
  • Fatty Acids / analysis
  • Hydrogen-Ion Concentration
  • Nitrogen / analysis
  • Phosphates / analysis
  • Photoperiod
  • Seasons*
  • Temperature
  • Waste Disposal, Fluid / instrumentation
  • Waste Disposal, Fluid / methods*
  • Wastewater / chemistry*
  • gamma-Aminobutyric Acid / analysis

Substances

  • Amino Acids
  • Fatty Acids
  • Phosphates
  • Waste Water
  • Carbon Dioxide
  • gamma-Aminobutyric Acid
  • Nitrogen
  • Acetic Acid