A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra

Carole Gardiennet; Anne K Schütz; Andreas Hunkeler; Britta Kunert; Laurent Terradot; Anja Böckmann; Beat H Meier

doi:10.1002/anie.201200779

A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra

Angew Chem Int Ed Engl. 2012 Jul 27;51(31):7855-8. doi: 10.1002/anie.201200779. Epub 2012 Jun 27.

Authors

Carole Gardiennet¹, Anne K Schütz, Andreas Hunkeler, Britta Kunert, Laurent Terradot, Anja Böckmann, Beat H Meier

Affiliation

¹ Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon, France.

PMID: 22740125
DOI: 10.1002/anie.201200779

Abstract

Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DnaB Helicases / chemistry*
DnaB Helicases / metabolism
Nuclear Magnetic Resonance, Biomolecular*

Substances

DnaB Helicases