Cystoisospora spp. from dogs in China and phylogenetic analysis of its 18S and ITS1 gene

Vet Parasitol. 2012 Nov 23;190(1-2):254-8. doi: 10.1016/j.vetpar.2012.05.025. Epub 2012 Jun 1.

Abstract

Cystoisospora spp. oocysts isolated from dog feces in Changchun, China were morphologically similar to those of Cystoisospora ohioensis and Cystoisospora sp. 1-MM recently isolated from dogs in Japanese. Sequencing results of the 18S subunit RNA gene from isolates in the present study were compared to other Cystoisospora spp. and the results suggested that Cystoisospora spp. from dogs in Changchun was homologous to C. ohioensis and Cystoisospora sp. 1-MM. Phylogenetic analysis of the 18S rRNA sequences showed that the Cystoisospora sp. ChangChun 1 and Cystoisospora sp. ChangChun 2 were nested in a clade with other Cystoisospora spp., including C. ohioensis, Cystoisospora belli, Cystoisospora suis, Isospora sp. Harbin/01/08 and C. orlovi,. Cystoisospora sp. ChangChun 2 was confirmed as C. ohioensis, and the other isolate was in a separate clade but the genetic relationship was relatively close to C. suis after analysis of the ITS-1gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • China
  • Coccidiosis / diagnosis
  • Coccidiosis / parasitology
  • Coccidiosis / veterinary*
  • DNA, Protozoan / chemistry
  • DNA, Protozoan / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics*
  • Dog Diseases / diagnosis
  • Dog Diseases / parasitology*
  • Dogs
  • Feces / parasitology
  • Phylogeny
  • RNA, Ribosomal, 18S / genetics*
  • Sarcocystidae / classification
  • Sarcocystidae / genetics*
  • Sequence Analysis, DNA

Substances

  • DNA, Protozoan
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 18S