Caffeoylserotonin suppresses THP-1 monocyte adhesion and migration via inhibition of the integrin β1/FAK/Akt signalling pathway

Fitoterapia. 2012 Dec;83(8):1364-70. doi: 10.1016/j.fitote.2012.04.025. Epub 2012 May 2.

Abstract

We investigated the effect of caffeoylserotonin (CaS) on THP-1 monocyte migration and adhesion to fibronectin in response to MCP-1. CaS decreased monocyte adhesion and migration induced by MCP-1, together with CCR2 expression and α5β1 integrin, and activated β1 integrin expression on the cell surface. CaS also inhibited FAK and Akt phosphorylation. We found that CaS had anti-inflammatory activity based on inhibition of adhesion and migration via inhibition of the integrin β1/FAK/Akt signalling pathway. Thus, the inhibitory effects of CaS on monocyte function may support the future development of this compound as a potential treatment for inflammation-dependent diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Caffeic Acids / chemistry
  • Caffeic Acids / pharmacology*
  • Cell Adhesion / drug effects*
  • Cell Movement / drug effects
  • Gene Expression Regulation
  • Integrin alpha5beta1 / genetics
  • Integrin alpha5beta1 / metabolism*
  • Monocytes / drug effects*
  • Monocytes / physiology
  • Proto-Oncogene Proteins c-akt / genetics
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Receptors, CCR2 / genetics
  • Receptors, CCR2 / metabolism
  • Serotonin / analogs & derivatives*
  • Serotonin / chemistry
  • Serotonin / pharmacology*
  • Signal Transduction

Substances

  • CCR2 protein, human
  • Caffeic Acids
  • Integrin alpha5beta1
  • N-caffeoylserotonin
  • Receptors, CCR2
  • Serotonin
  • Proto-Oncogene Proteins c-akt