Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the edible dormouse, Glis glis

H Hürner; J F Martin; A Ribas; A Arrizabalaga; J R Michaux

doi:10.1111/j.1755-0998.2008.02365.x

Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the edible dormouse, Glis glis

Mol Ecol Resour. 2009 May;9(3):885-7. doi: 10.1111/j.1755-0998.2008.02365.x. Epub 2009 Feb 5.

Authors

H Hürner¹, J F Martin, A Ribas, A Arrizabalaga, J R Michaux

Affiliation

¹ Laboratoire de génétique des microorganismes, University of Liège, Bât. B22, 27 Boulevard du Rectorat, Sart Tilman, 4000 Liège, Belgium, UMR 1062 CBGP, Campus international de Baillarguet, CS30016, F-34988, Montferrier sur Lez cedex, France, INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988 Montferrier-sur-Lez cedex, France, Museu de Granollers-Ciències Naturals C/Francesc Macià, 51 08402 Granollers.

PMID: 21564778
DOI: 10.1111/j.1755-0998.2008.02365.x

Abstract

We isolated and characterized 10 dinucleotide microsatellite loci in the edible dormouse, Glis glis (Linnaeus). Four multiplex panels were developed. Loci were amplified in samples from two geographically distant populations (Torgny in Belgium and Montseny in Spain). All loci were polymorphic in Spain but four were monomorphic in Belgium. Individuals from Belgium and Spain exhibited an average allelic diversity of 1.9 and 3.3 and an observed heterozygosity ranging from 0.08 to 0.47 and from 0.04 to 0.72, respectively.