The removal of chromium (VI) ions from aqueous solutions by the biomass of two marine strains of Yarrowia lipolytica (NCIM 3589 and 3590) was studied with respect to pH, temperature, biomass, sea salt concentration, agitation speed, contact time and initial concentration of chromium (VI) ions. Maximum biosorption was observed at pH 1.0 and at a temperature of 35 degrees C. Increase in biomass and sea salts resulted in a decreased metal uptake. With an agitation speed of 130 rpm, equilibrium was attained within 2h. Under optimum conditions, biosorption was enhanced with increasing concentrations of Cr (VI) ions. NCIM 3589 and 3590 displayed a specific uptake of Cr (VI) ions of 63.73+/-1.3 mg g(-1) at a concentration of 950 ppm and 46.09+/-0.23 mg g(-1) at 955 ppm, respectively. Scatchard plot analysis revealed a straight line allowing the data to be fitted in the Langmuir model. The adsorption data obtained also fitted well to the Freundlich isotherm. The surface sequestration of Cr (VI) by Y. lipolytica was investigated with a scanning electron microscope equipped with an energy dispersive spectrometer (SEM-EDS) as well as with ED-X-ray fluorescence (ED-XRF). Fourier transform infrared (FTIR) spectroscopy revealed the involvement of carboxyl, hydroxyl and amide groups on the cell surfaces in chromium binding.