Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances

Hisatoshi Kaneko; Takashi Kawana; Eiko Fukushima; Tatsuo Suzutani

doi:10.1016/j.jbbm.2006.08.008

Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances

J Biochem Biophys Methods. 2007 Apr 10;70(3):499-501. doi: 10.1016/j.jbbm.2006.08.008. Epub 2006 Aug 30.

Authors

Hisatoshi Kaneko¹, Takashi Kawana, Eiko Fukushima, Tatsuo Suzutani

Affiliation

¹ Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, 960-1295, Japan.

PMID: 17011631
DOI: 10.1016/j.jbbm.2006.08.008

Abstract

To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chlorocebus aethiops
Culture Media
DNA, Viral / genetics
DNA, Viral / isolation & purification
Herpesvirus 1, Human / genetics
Herpesvirus 1, Human / isolation & purification
Humans
Microbiological Techniques / methods*
Microbiological Techniques / statistics & numerical data
Nucleic Acid Amplification Techniques / methods*
Nucleic Acid Amplification Techniques / statistics & numerical data
Polymerase Chain Reaction
Sensitivity and Specificity
Vero Cells

Substances

Culture Media
DNA, Viral