Organization of the replication control region of plasmid ColIb-P9

J Bacteriol. 1990 Apr;172(4):1983-91. doi: 10.1128/jb.172.4.1983-1991.1990.

Abstract

We identified a 1,845-base-pair sequence that contains essential information for the autonomous replication and regulation of the 93-kilobase-pair IncI alpha group ColIb-P9 plasmid. Biochemical and genetic analyses revealed that this sequence specifies at least two structural genes, designated repZ and inc. The repZ gene encodes a protein with a molecular weight of 39,000, which probably functions as an initiator for the ColIb-P9 replicon. The inc gene that phenotypically governs the incompatibility encodes an RNA with a size of about 70 bases. This small RNA acts in trans to repress the expression of repZ, thereby functioning to maintain a constant copy number of the ColIb-P9 replicon in host cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Coliphages / genetics
  • DNA Replication*
  • DNA, Bacterial / genetics*
  • DNA-Binding Proteins / genetics
  • Escherichia coli / genetics*
  • Genes, Bacterial
  • Genotype
  • Molecular Sequence Data
  • Mutation
  • Plasmids*
  • RNA, Bacterial / genetics
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Transcription, Genetic
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • RNA, Bacterial
  • RepZ protein, plasmid ColIb-P9
  • beta-Galactosidase

Associated data

  • GENBANK/M34837