Abstract
Protocols for modifying gold nanoparticles with peptide-bovine serum albumin (BSA) conjugates are described within. The resulting constructs were characterized using a number of techniques including static fluorescence spectroscopy and time-correlated single photon counting spectroscopy (TCSPC) in order to quantify peptide-BSA binding isotherms, exchange rates, critical flocculation concentrations, and the composition of mixed peptide-BSA monolayers on gold nanoparticles. TCSPC has proven to be a powerful technique for observing the microenvironment of protein-gold nanoparticle conjugates because it can distinguish between surface-bound and solution-phase species without the need for separation steps. Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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2,2'-Dipyridyl / analogs & derivatives*
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2,2'-Dipyridyl / chemistry
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Cattle
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Coordination Complexes
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Dithionitrobenzoic Acid / chemistry
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Flocculation
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Fluorescamine / chemistry
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Glutathione / chemistry
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Gold Colloid / chemistry*
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Gold Colloid / metabolism
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Kinetics
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Ligands
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Nanotechnology / methods*
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Particle Size
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Peptides / chemistry*
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Protein Binding
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Rhodamines / chemistry
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Serum Albumin, Bovine / chemistry
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Serum Albumin, Bovine / metabolism
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Spectrometry, Fluorescence
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Spectrophotometry
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Spectrophotometry, Ultraviolet
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Succinimides / chemistry
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Sulfhydryl Compounds / chemistry
Substances
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Coordination Complexes
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Gold Colloid
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Ligands
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Peptides
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Rhodamines
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Succinimides
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Sulfhydryl Compounds
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tris(2,2-bipyridine)-ruthenium(II)
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Serum Albumin, Bovine
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Fluorescamine
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2,2'-Dipyridyl
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3-maleimidobenzoyl N-hydroxysuccinimide
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Dithionitrobenzoic Acid
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rhodamine isothiocyanate
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Glutathione