Abstract
The Brucella melitensis sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme (previously identified as an immunogenic protein in infected sheep) was cloned and sequenced. The amino acid sequence predicted from the cloned gene revealed 88.8 and 51.2% identity to the dihydrolipoamide succinyltransferase SucB protein from Brucella abortus and Escherichia coli, respectively. Sera from naturally infected sheep showed antibody reactivity against the recombinant SucB protein.
MeSH terms
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Acyltransferases / genetics*
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Acyltransferases / immunology
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Animals
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Antibodies, Bacterial / immunology
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Antibodies, Monoclonal / immunology
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Antibody Specificity
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Base Sequence
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Brucella melitensis / enzymology*
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Brucella melitensis / genetics
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Brucellosis / blood
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Brucellosis / immunology
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Brucellosis / veterinary*
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Cloning, Molecular
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DNA, Bacterial
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Escherichia coli
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Gene Expression
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Genes, Bacterial
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Molecular Sequence Data
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Sequence Analysis, DNA
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Sheep
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Sheep Diseases / blood
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Sheep Diseases / immunology*
Substances
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Antibodies, Bacterial
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Antibodies, Monoclonal
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DNA, Bacterial
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Acyltransferases
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dihydrolipoamide succinyltransferase