Expression and localization of the CDC34 ubiquitin-conjugating enzyme in pediatric acute lymphoblastic leukemia

E Eliseeva; D Pati; M B Diccinanni; A L Yu; S K Mohsin; J F Margolin; S E Plon

Expression and localization of the CDC34 ubiquitin-conjugating enzyme in pediatric acute lymphoblastic leukemia

Cell Growth Differ. 2001 Aug;12(8):427-33.

Authors

E Eliseeva¹, D Pati, M B Diccinanni, A L Yu, S K Mohsin, J F Margolin, S E Plon

Affiliation

¹ Department of Pediatrics, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas 77030, USA.

PMID: 11504708

Abstract

Ubiquitin-dependent protein degradation impacts many cellular processes.However, the regulation of ubiquitin-conjugating enzymes (UBCs) in cancer is unknown. We find that the human CDC34 UBC protein is expressed at a 3-4 fold higher level (P < 0.001) in pediatric T cell than in pre-B-cell acute lymphoblastic leukemia (ALL) before treatment in two independent patient sets. The level of CDC34 mRNA was similar in both types of leukemia. CDC34 expression levels in normal resting T cells, B cells and activated T lymphocytes was comparable with pre-B-cell ALL. CDC34 protein (but not mRNA) was also increased in T-cell ALL compared with pre-B-cell ALL cell lines. The difference in expression was not attributable to mutation or associated with altered CDC34 stability. Immunohistochemistry and cellular fractionation reveals a heterogeneous CDC34 expression pattern including cells containing primarily cytoplasmic or nuclear protein. Thus, a feature of pediatric T-cell ALL is posttranscriptional up-regulation and heterogeneous localization of the human CDC34 UBC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anaphase-Promoting Complex-Cyclosome
Burkitt Lymphoma / enzymology
Burkitt Lymphoma / pathology
Burkitt Lymphoma / physiopathology
Cell Division / physiology
Child
Gene Expression Regulation, Leukemic / physiology*
Humans
Immunohistochemistry
Leukemia-Lymphoma, Adult T-Cell / enzymology
Leukemia-Lymphoma, Adult T-Cell / pathology
Leukemia-Lymphoma, Adult T-Cell / physiopathology
Ligases / genetics*
Ligases / metabolism*
Lymphocyte Activation / physiology
Mutation / physiology
Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
Precursor Cell Lymphoblastic Leukemia-Lymphoma / physiopathology
RNA, Messenger / metabolism
Tumor Cells, Cultured / cytology
Tumor Cells, Cultured / enzymology
Ubiquitin-Conjugating Enzymes
Ubiquitin-Protein Ligase Complexes*
Ubiquitin-Protein Ligases
Ubiquitins / metabolism*

Substances

RNA, Messenger
Ubiquitins
CDC34 protein, human
Ubiquitin-Conjugating Enzymes
Ubiquitin-Protein Ligase Complexes
Anaphase-Promoting Complex-Cyclosome
Ubiquitin-Protein Ligases
Ligases