Cloning, expression, and function of BLAME, a novel member of the CD2 family

G A Kingsbury; L A Feeney; Y Nong; S A Calandra; C J Murphy; J M Corcoran; Y Wang; M R Prabhu Das; S J Busfield; C C Fraser; J L Villeval

doi:10.4049/jimmunol.166.9.5675

Cloning, expression, and function of BLAME, a novel member of the CD2 family

J Immunol. 2001 May 1;166(9):5675-80. doi: 10.4049/jimmunol.166.9.5675.

Authors

G A Kingsbury¹, L A Feeney, Y Nong, S A Calandra, C J Murphy, J M Corcoran, Y Wang, M R Prabhu Das, S J Busfield, C C Fraser, J L Villeval

Affiliation

¹ Millennium Pharmaceutical, Cambridge, MA 02139, USA. kingsbury@mpi.com

PMID: 11313408
DOI: 10.4049/jimmunol.166.9.5675

Abstract

The CD2 family is a growing family of Ig domain-containing cell surface proteins involved in lymphocyte activation. Here we describe the cloning and expression analysis of a novel member of this family, B lymphocyte activator macrophage expressed (BLAME). BLAME shares the structural features of the CD2 family containing an IgV and IgC2 domain and clusters with the other family members on chromosome 1q21. Quantitative PCR and Northern blot analysis show BLAME to be expressed in lymphoid tissue and, more specifically, in some populations of professional APCs, activated monocytes, and DCS: Retroviral forced expression of BLAME in hematopoietic cells of transplanted mice showed an increase in B1 cells in the peripheral blood, spleen, lymph nodes, and, most strikingly, in the peritoneal cavity. These cells do not express CD5 and are CD23(low)Mac1(low), characteristics of the B1b subset. BLAME may therefore play a role in B lineage commitment and/or modulation of signal through the B cell receptor.

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Animals
Antigen-Presenting Cells / immunology
Antigen-Presenting Cells / metabolism
Antigens, Differentiation, B-Lymphocyte / biosynthesis
Antigens, Differentiation, B-Lymphocyte / genetics*
Antigens, Differentiation, B-Lymphocyte / physiology
Bone Marrow Transplantation / immunology
CD2 Antigens* / genetics
Cells, Cultured
Cloning, Molecular
Dendritic Cells / immunology
Dendritic Cells / metabolism
Humans
Macrophages / immunology
Macrophages / metabolism
Membrane Proteins / biosynthesis
Membrane Proteins / genetics*
Membrane Proteins / physiology
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Multigene Family / immunology*
Organ Specificity / genetics
Organ Specificity / immunology
Radiation Chimera / immunology
Retroviridae / genetics
Sequence Homology, Amino Acid*
Signaling Lymphocytic Activation Molecule Family
Transduction, Genetic

Substances

Antigens, Differentiation, B-Lymphocyte
CD2 Antigens
Membrane Proteins
SLAMF8 protein, human
Signaling Lymphocytic Activation Molecule Family