The rpoN gene of Enterococcus faecalis directs sensitivity to subclass IIa bacteriocins

K Dalet; C Briand; Y Cenatiempo; Y Héchard

doi:10.1007/s002840010164

The rpoN gene of Enterococcus faecalis directs sensitivity to subclass IIa bacteriocins

Curr Microbiol. 2000 Dec;41(6):441-3. doi: 10.1007/s002840010164.

Authors

K Dalet¹, C Briand, Y Cenatiempo, Y Héchard

Affiliation

¹ Laboratoire de Biologie Moléculaire, CNRS ESA 6031, IBMIG, Université de Poitiers, 40 avenue du recteur Pineau, 86022 Poitiers Cedex, France.

PMID: 11080395
DOI: 10.1007/s002840010164

Abstract

The final sigma 54 factor has been previously described to be involved in Listeria monocytogenes sensitivity to mesentericin Y105, a subclass IIa bacteriocin. Here, we identified the rpoN gene, encoding final sigma 54, of Enterococcus faecalis JH2-2 and showed that its interruption leads to E. faecalis resistance to different subclass IIa bacteriocins. Moreover, this rpoN mutant remained sensitive to nisin, a class I bacteriocin, suggesting that final sigma 54 is especially involved in sensitivity to subclass IIa bacteriocins.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Bacteriocins / classification
Bacteriocins / pharmacology*
DNA-Binding Proteins*
DNA-Directed RNA Polymerases / genetics*
Drug Resistance, Microbial / genetics
Enterococcus faecalis / drug effects*
Enterococcus faecalis / genetics*
Genes, Bacterial
Genetic Complementation Test
Microbial Sensitivity Tests
Molecular Sequence Data
Mutagenesis, Insertional
RNA Polymerase Sigma 54
Restriction Mapping
Sequence Analysis, DNA
Sigma Factor / genetics*

Substances

Bacteriocins
DNA-Binding Proteins
Sigma Factor
DNA-Directed RNA Polymerases
RNA Polymerase Sigma 54

Associated data

GENBANK/AF210458