Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12

A Kato; H Ohnishi; K Yamamoto; E Furuta; H Tanabe; R Utsumi

doi:10.1271/bbb.64.1203

Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12

Biosci Biotechnol Biochem. 2000 Jun;64(6):1203-9. doi: 10.1271/bbb.64.1203.

Authors

A Kato¹, H Ohnishi, K Yamamoto, E Furuta, H Tanabe, R Utsumi

Affiliation

¹ Department of Agricultural Chemistry, Kinki University, Nara, Japan.

PMID: 10923791
DOI: 10.1271/bbb.64.1203

Abstract

Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgSI and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.

MeSH terms

Amino Acid Sequence
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Base Sequence
Binding Sites / genetics
DNA Primers / genetics
DNA, Bacterial / genetics
DNA, Bacterial / metabolism
Drug Resistance, Microbial / genetics
Escherichia coli / genetics*
Escherichia coli / metabolism*
Escherichia coli Proteins*
Genes, Bacterial*
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
Transcription Factors / genetics*
Transcription Factors / metabolism*
Transcription, Genetic

Substances

Bacterial Proteins
BvgA protein, Bacteria
DNA Primers
DNA, Bacterial
Escherichia coli Proteins
EvgA protein, E coli
Transcription Factors
bvgS protein, Bordetella pertussis

Associated data

GENBANK/AE000325
GENBANK/AF201840
GENBANK/AF201841
GENBANK/D78168