Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-I

Nat Cell Biol. 1999 Jul;1(3):165-70. doi: 10.1038/11086.

Abstract

Calcium plays a critical part in the regulation of cell growth, and growth factors stimulate calcium entry into cells through calcium-permeable channels. However, the molecular nature and regulation of calcium-permeable channels are still unclear at present. Here we report the molecular characterization of a calcium-permeable cation channel that is regulated by insulin-like growth factor-I (IGF-I). This channel, which we name growth-factor-regulated channel (GRC), belongs to the TRP-channel family and localizes mainly to intracellular pools under basal conditions. Upon stimulation of cells by IGF-I, GRC translocates to the plasma membrane. Thus, IGF-I augments calcium entry through GRC by regulating trafficking of the channel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Androstadienes / pharmacology
  • Animals
  • Calcium / metabolism*
  • Calcium Channels / chemistry
  • Calcium Channels / genetics
  • Calcium Channels / physiology*
  • Cattle
  • Cell Membrane / physiology*
  • Chromones / pharmacology
  • Cloning, Molecular
  • Cytosol / metabolism
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Humans
  • Insulin-Like Growth Factor I / pharmacology*
  • Insulin-Like Growth Factor I / physiology
  • Membrane Potentials / drug effects
  • Mice
  • Molecular Sequence Data
  • Morpholines / pharmacology
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phylogeny
  • Rats
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • TRPV Cation Channels
  • Transfection
  • Wortmannin

Substances

  • Androstadienes
  • Calcium Channels
  • Chromones
  • Morpholines
  • Recombinant Fusion Proteins
  • TRPV Cation Channels
  • TRPV2 protein, human
  • Trpv2 protein, mouse
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Insulin-Like Growth Factor I
  • Phosphatidylinositol 3-Kinases
  • Calcium
  • Wortmannin

Associated data

  • GENBANK/AB021665