Improved detection of HIV-2 DNA in clinical samples using a nested primer-based polymerase chain reaction

J Acquir Immune Defic Syndr (1988). 1992;5(3):286-93.

Abstract

A two-step polymerase chain reaction (PCR), with four double (nested) primer pairs, used for the detection of HIV-2 in clinical samples is described. With these four nested primer pairs we could detect HIV-2 DNA in 17 of 17 virus isolates and in blood mononuclear cell samples from 31 of 37 (83.7%) seropositive individuals after ethidium bromide staining of the amplified DNA. The nested primer PCR was also compared with a single primer pair-based PCR followed by hybridization. The sensitivities of the two methods were almost equal, but the nested primer PCR offered obvious technical advantages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Base Composition
  • Base Sequence
  • DNA Probes
  • DNA, Viral / blood*
  • Female
  • Gene Amplification
  • HIV-2 / genetics*
  • Humans
  • Leukocytes, Mononuclear / chemistry
  • Macaca
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction* / methods
  • Polymerase Chain Reaction* / standards
  • Simian Immunodeficiency Virus / genetics
  • Virus Integration

Substances

  • DNA Probes
  • DNA, Viral