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A model for improving microbial biofuel production using a synthetic feedback loop.

Dunlop MJ, Keasling JD, Mukhopadhyay A.

Syst Synth Biol. 2010 Jun;4(2):95-104. doi: 10.1007/s11693-010-9052-5. Epub 2010 Feb 25.


Microbial biosynthesis of alkanes.

Schirmer A, Rude MA, Li X, Popova E, del Cardayre SB.

Science. 2010 Jul 30;329(5991):559-62. doi: 10.1126/science.1187936.


Computational design of an enzyme catalyst for a stereoselective bimolecular Diels-Alder reaction.

Siegel JB, Zanghellini A, Lovick HM, Kiss G, Lambert AR, St Clair JL, Gallaher JL, Hilvert D, Gelb MH, Stoddard BL, Houk KN, Michael FE, Baker D.

Science. 2010 Jul 16;329(5989):309-13. doi: 10.1126/science.1190239.


Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control.

Leonard E, Ajikumar PK, Thayer K, Xiao WH, Mo JD, Tidor B, Stephanopoulos G, Prather KL.

Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13654-9. doi: 10.1073/pnas.1006138107. Epub 2010 Jul 19.


Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisiae.

Siewers V, San-Bento R, Nielsen J.

Biotechnol Bioeng. 2010 Aug 1;106(5):841-4. doi: 10.1002/bit.22739.


Creation of a bacterial cell controlled by a chemically synthesized genome.

Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.


Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.

Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD.

Nature. 2010 Jan 28;463(7280):559-62. doi: 10.1038/nature08721.


Genes involved in long-chain alkene biosynthesis in Micrococcus luteus.

Beller HR, Goh EB, Keasling JD.

Appl Environ Microbiol. 2010 Feb;76(4):1212-23. doi: 10.1128/AEM.02312-09. Epub 2009 Dec 28.


Automated design of synthetic ribosome binding sites to control protein expression.

Salis HM, Mirsky EA, Voigt CA.

Nat Biotechnol. 2009 Oct;27(10):946-50. doi: 10.1038/nbt.1568. Epub 2009 Oct 4.


Programming cells by multiplex genome engineering and accelerated evolution.

Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM.

Nature. 2009 Aug 13;460(7257):894-8. doi: 10.1038/nature08187. Epub 2009 Jul 26.


A novel semi-biosynthetic route for artemisinin production using engineered substrate-promiscuous P450(BM3).

Dietrich JA, Yoshikuni Y, Fisher KJ, Woolard FX, Ockey D, McPhee DJ, Renninger NS, Chang MC, Baker D, Keasling JD.

ACS Chem Biol. 2009 Apr 17;4(4):261-7. doi: 10.1021/cb900006h.


Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains.

Wisselink HW, Toirkens MJ, Wu Q, Pronk JT, van Maris AJ.

Appl Environ Microbiol. 2009 Feb;75(4):907-14. doi: 10.1128/AEM.02268-08. Epub 2008 Dec 12.


Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol.

Steen EJ, Chan R, Prasad N, Myers S, Petzold CJ, Redding A, Ouellet M, Keasling JD.

Microb Cell Fact. 2008 Dec 3;7:36. doi: 10.1186/1475-2859-7-36.


De novo biosynthetic pathways: rational design of microbial chemical factories.

Prather KL, Martin CH.

Curr Opin Biotechnol. 2008 Oct;19(5):468-74. doi: 10.1016/j.copbio.2008.07.009. Epub 2008 Sep 5. Review.


Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae.

Hawkins KM, Smolke CD.

Nat Chem Biol. 2008 Sep;4(9):564-73. doi: 10.1038/nchembio.105.


Application of functional genomics to pathway optimization for increased isoprenoid production.

Kizer L, Pitera DJ, Pfleger BF, Keasling JD.

Appl Environ Microbiol. 2008 May;74(10):3229-41. doi: 10.1128/AEM.02750-07. Epub 2008 Mar 14.


Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels.

Atsumi S, Hanai T, Liao JC.

Nature. 2008 Jan 3;451(7174):86-9. doi: 10.1038/nature06450.


Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity.

Withers ST, Gottlieb SS, Lieu B, Newman JD, Keasling JD.

Appl Environ Microbiol. 2007 Oct;73(19):6277-83. Epub 2007 Aug 10.


Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation.

Park JH, Lee KH, Kim TY, Lee SY.

Proc Natl Acad Sci U S A. 2007 May 8;104(19):7797-802. Epub 2007 Apr 26.


Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.

Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD.

Nat Chem Biol. 2007 May;3(5):274-7. Epub 2007 Apr 15.

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