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Biochim Biophys Acta. 1976 Nov 26;453(1):151-61.

Formiminotransferase cyclodeaminase from porcine liver. An octomeric enzyme containing bifunctional polypeptides.

Abstract

The forminiminotransferase-cyclodeaminase complex contains 2.8% carbohydrate and is composed of identical subunits based on molecular weight, isoelectric focusing and the number of CNBr-generated peptides. Electron microscopy demonstrated that the complex is a ring-shaped molecule when negatively stained both in solution and in crystalline structures. Rotational reinforcement of electron micrographs reveals that the molecule is composed of eight subunits arranged in a planar ring. These results indicate that each polypeptide of the complex must contain both enzymic activities.

PMID:
999878
[PubMed - indexed for MEDLINE]
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