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Biochim Biophys Acta. 1999 Jan 11;1429(2):439-45.

Purification and characterization of a new enzyme, N-alkylglycine oxidase from Cladosporium sp. G-10.

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  • 1Research and Development Division, Kikkoman Corporation, Chiba, Japan. 8553@mail.kikkoman.co.jp

Abstract

A new enzyme, N-alkylglycine oxidase, was isolated from a soil mold, Cladosporium sp. G-10. This protein, which was purified to near homogeneity by ammonium sulfate precipitation followed by successive column chromatography on phenyl-Sepharose, DEAE-Sepharose and Sephadex G-200, was a single polypeptide with a molecular mass of 52,000. In the presence of O2 and H2O, this enzyme acted on some N-alkylglycine derivatives, such as N epsilon-carboxymethyllysine, N-carboxymethyl-6-aminocaproic acid, sarcosine and N-ethylglycine, and produced corresponding N-alkylamine, glyoxylic acid and H2O2. This enzyme had optimum activity at 30 degrees C, pH 8-10, and was most inhibited by ZnSO4, pCMB, iodoacetic acid, and SDS.

PMID:
9989229
[PubMed - indexed for MEDLINE]
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