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Fertil Steril. 1999 Feb;71(2):361-7.

Human endometrial stromal cells improve embryo quality by enhancing the expression of insulin-like growth factors and their receptors in cocultured human preimplantation embryos.

Author information

  • 1The Center for Reproductive Medicine and Infertility, The New York Hospital-Cornell Medical Center, New York 10021, USA. hcliu@mail.med.cornell.edu

Abstract

OBJECTIVE:

To demonstrate the mechanism by which human endometrial stromal cells improve embryo quality in coculture.

DESIGN:

Randomized study.

SETTING:

Academic research center.

PATIENT(S):

Patients undergoing IVF-ET.

INTERVENTION(S):

Donated human embryos were cultured randomly either alone (group A) or with human endometrial stromal cells (group B), and the embryonic expression of insulin-like growth factors (IGFs) and their receptors was detected by reverse transcriptase polymerase chain reaction after culture.

MAIN OUTCOME MEASURE(S):

The embryo frequency distribution of groups A and B before and after culture and the embryonic transcripts of the IGF family genes of the two study groups after culture were compared.

RESULT(S):

The embryo frequency distribution of the day 3 embryonic stages in groups A and B was not different. However, after culture, a statistically significant difference in blastocyst formation was observed between groups A and B. A significant increase in the expression of IGF-1, IGF-2, the IGF-1 receptor, and the insulin-receptor also was noted. Among the embryos that reached the blastocyst stage, the expression of IGF-1 and the IGF-1 receptor also was significantly different in the two study groups.

CONCLUSION(S):

Human endometrial stromal cells enhanced the expression of IGFs and their receptors in cocultured human embryos, which may be essential for improving embryo quality.

PMID:
9988412
[PubMed - indexed for MEDLINE]
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