Detection of light subunit neurofilament and glial fibrillary acidic protein in cerebrospinal fluid of Trypanosoma brucei gambiense-infected patients

Am J Trop Med Hyg. 1999 Jan;60(1):94-8. doi: 10.4269/ajtmh.1999.60.94.

Abstract

Light subunit neurofilament (NFL) and glial fibrillary acidic protein (GFAP) concentrations were determined in cerebrospinal fluid (CSF) of 34 patients with human African trypanosomiasis (HAT), five serologically positive but parasitologically unconfirmed individuals, and four healthy controls without evidence of HAT. In patients with second stage HAT (n = 30), NFL levels were abnormally elevated in 10 cases and GFAP levels in five. The astrogliosis observed in HAT and experimental models of HAT is confirmed in our study by the presence of increased GFAP levels in the CSE The abnormal NFL CSF levels reflect structural damage of nerve cells in 33 % of the second-stage patients studied. To our knowledge, this is the first time neuronal damage in HAT patients is demonstrated by using biochemical markers of brain damage in the CSF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Aged
  • Animals
  • Biomarkers / cerebrospinal fluid
  • Child
  • Disease Progression
  • Glial Fibrillary Acidic Protein / cerebrospinal fluid*
  • Humans
  • Middle Aged
  • Neurofilament Proteins / cerebrospinal fluid*
  • Trypanosoma brucei gambiense*
  • Trypanosomiasis, African / cerebrospinal fluid*
  • Trypanosomiasis, African / diagnosis
  • Trypanosomiasis, African / pathology

Substances

  • Biomarkers
  • Glial Fibrillary Acidic Protein
  • Neurofilament Proteins
  • neurofilament protein L