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Genomics. 1999 Jan 15;55(2):248-51.

Assignment of the melatonin-related receptor to human chromosome X (GPR50) and mouse chromosome X (Gpr50).

Author information

  • 1Pediatric Service, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, 02114, USA.

Abstract

Recent efforts to clone further members of the melatonin receptor family have led to the identification of a novel G-protein-coupled receptor in human pituitary. This receptor, referred to as H9, is clearly related to high-affinity melatonin receptors yet unable to bind this hormone. We now report the cloning and expression of the cDNA encoding the H9 receptor in mice. The mouse clone encodes a protein of 591 amino acids that shares 74% amino acid identity with the human receptor and is unable to bind 2-[125I]iodomelatonin when transiently expressed in COS-7 cells. We also determined the chromosome loci of the human and mouse H9 receptor genes. Both genes were found to be X-linked: radiation hybrid mapping revealed that the human H9 gene (GPR50) is localized to Xq28. The mouse gene (Gpr50) was determined to lie in the proximal portion of chromosome X by means of interspecific backcross analysis. These loci might be relevant to genetically based neuroendocrine disorders.

Copyright 1999 Academic Press.

PMID:
9933574
[PubMed - indexed for MEDLINE]

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