T lymphocyte costimulation mediated by reorganization of membrane microdomains

Science. 1999 Jan 29;283(5402):680-2. doi: 10.1126/science.283.5402.680.

Abstract

Although dispensable, costimulation through CD28 facilitates activation of naïve T lymphocytes. CD28 engagement led to the redistribution and clustering of membrane and intracellular kinase-rich raft microdomains at the site of T cell receptor (TCR) engagements. Although not affecting TCR down-regulation, this process led to higher and more stable tyrosine phosphorylation of several substrates and higher consumption of Lck. These results may provide a general mechanism for amplifying receptor signaling by reorganization of membrane microdomains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen-Presenting Cells / immunology
  • CD28 Antigens / immunology
  • CD28 Antigens / metabolism*
  • CD3 Complex / immunology
  • Cell Membrane / metabolism
  • G(M1) Ganglioside / metabolism
  • Humans
  • Lymphocyte Activation*
  • Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / metabolism
  • Membrane Lipids / metabolism*
  • Phosphorylation
  • Phosphotyrosine / metabolism
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell / metabolism*
  • Signal Transduction
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism

Substances

  • CD28 Antigens
  • CD3 Complex
  • Membrane Lipids
  • Receptors, Antigen, T-Cell
  • Phosphotyrosine
  • G(M1) Ganglioside
  • Lymphocyte Specific Protein Tyrosine Kinase p56(lck)