Abstract
The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane II-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three receptors were identified to respond to micromolecular concentrations of carvone, (-) citronellal, and limonene, respectively. We also found that the mouse I7 receptor, unlike the rat I7 receptor, prefers heptanal instead of octanal, as a result of a single valine-to-isoleucine substitution. This finding represents the beginning of a molecular understanding of odorant recognition. The identification, on a large scale, of cognate receptor-odorant interactions should provide insight into olfactory coding mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholine / pharmacology
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Acyclic Monoterpenes
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Aldehydes / metabolism
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Aldehydes / pharmacology
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Amino Acid Substitution
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Animals
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Binding Sites
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Calcium Signaling
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Cell Line
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Cell Membrane / metabolism
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Cloning, Molecular* / methods
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Down-Regulation
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Fatty Alcohols / metabolism
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Fatty Alcohols / pharmacology
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Isoproterenol / pharmacology
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Ligands
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Mice
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Molecular Sequence Data
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Monoterpenes*
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Peptide Library
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Rats
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Receptors, Adrenergic, beta / genetics
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Receptors, Adrenergic, beta / metabolism
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Receptors, Odorant / chemistry
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Receptors, Odorant / genetics*
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Receptors, Odorant / metabolism*
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Recombinant Fusion Proteins / metabolism
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Rhodopsin / genetics
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Rhodopsin / metabolism
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Stereoisomerism
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Structure-Activity Relationship
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Terpenes / metabolism
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Terpenes / pharmacology
Substances
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Acyclic Monoterpenes
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Aldehydes
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Fatty Alcohols
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Ligands
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Monoterpenes
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Peptide Library
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Receptors, Adrenergic, beta
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Receptors, Odorant
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Recombinant Fusion Proteins
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Terpenes
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Rhodopsin
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Isoproterenol
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Acetylcholine
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citronellal
Associated data
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GENBANK/AF102516
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GENBANK/AF102517
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GENBANK/AF102518
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GENBANK/AF102519
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GENBANK/AF102537
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GENBANK/AF102538
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GENBANK/AF102539
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GENBANK/AF102540
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GENBANK/AF102541
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GENBANK/AF106007