Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):11-3.

A microfabricated device for sizing and sorting DNA molecules.

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Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.

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PMCID:: PMC15083

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