Abstract
P84 (also known as SHPS-1, BIT, and SIRP) is a heterophilic adhesive membrane protein involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. We have identified a binding partner for P84 using an expression cloning strategy. Here we report that integrin-associated protein (IAP/CD47) is a predominant binding partner of P84. Immunohistochemistry reveals a virtually identical distribution of P84 and IAP in a variety of adult brain regions. Because IAP has been implicated in cell signaling in cells of the immune system, P84 and IAP represent a heterophilic binding pair that is likely to be involved in bi-directional signaling at the synapse and in other tissues.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies / immunology
-
Antigens, CD / chemistry
-
Antigens, CD / immunology
-
Antigens, CD / metabolism*
-
Antigens, Differentiation*
-
Brain / metabolism
-
CD47 Antigen
-
Carrier Proteins / chemistry
-
Carrier Proteins / immunology
-
Carrier Proteins / metabolism*
-
Cloning, Molecular
-
DNA, Complementary
-
Ligands
-
Membrane Glycoproteins / metabolism*
-
Mice
-
Molecular Sequence Data
-
Neural Cell Adhesion Molecule L1*
-
Neural Cell Adhesion Molecules / metabolism*
-
Receptors, Immunologic*
-
Recombinant Fusion Proteins / metabolism
Substances
-
Antibodies
-
Antigens, CD
-
Antigens, Differentiation
-
CD47 Antigen
-
Carrier Proteins
-
Cd47 protein, mouse
-
DNA, Complementary
-
Ligands
-
Membrane Glycoproteins
-
Neural Cell Adhesion Molecule L1
-
Neural Cell Adhesion Molecules
-
Ptpns1 protein, mouse
-
Receptors, Immunologic
-
Recombinant Fusion Proteins