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Nat Biotechnol. 1998 Dec;16(13):1365-9.

The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids.

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  • 1Keygene N.V., Agro Business Park, Wageningen, The Netherlands. keygene@euronet.nl

Abstract

Mi-1, a Lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. Complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode Meloidogyne incognita and to an unrelated pathogen, the potato aphid Macrosiphum euphorbiae. This resistance was stable. The Mi-1 gene encodes a protein sharing structural features with the nucleotide-binding site leucine-rich repeat-containing type of plant resistance genes.

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PMID:
9853621
[PubMed - indexed for MEDLINE]
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