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Selection system for genes encoding nuclear-targeted proteins.
Biological Technology Laboratory, Helix Research Institute, Chiba, Japan. goose@rc.m-kagaku.co.jp
Nuclear proteins have essential roles in cell proliferation and differentiation. We have developed a yeast selection system-the nuclear transportation trap (NTT)-to identify genes encoding nuclear transport signals. Both unknown and previously identified nuclear localization signals were identified from a human fetal brain cDNA library. The majority (75%) of the unknown proteins examined were exclusively localized to the nucleus in COS-7 cells. We propose that NTT is an efficient method for isolating cDNAs that encode nuclear targeted proteins that can be applied to the retrieval of novel nuclear proteins and to annotate gene function.
PMID: 9853615 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection.
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[EMBO J. 2006]
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Functional isolation of novel nuclear proteins showing a variety of subnuclear localizations.
Moriguchi K, Suzuki T, Ito Y, Yamazaki Y, Niwa Y, Kurata N.
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[Plant Cell. 2005]
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Cell cycle-dependent nuclear localization of yeast RNase III is required for efficient cell division.
Catala M, Lamontagne B, Larose S, Ghazal G, Elela SA.
Mol Biol Cell. 2004 Jul; 15(7):3015-30. Epub 2004 Apr 16.
[Mol Biol Cell. 2004]
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