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Appl Environ Microbiol. 1998 Dec;64(12):5004-7.

Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi

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  • 1Centre de Recherche en Biologie Forestiere, Universite Laval, Quebec G1K 7P4, Canada.

Abstract

We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.

PMID:
9835596
[PubMed - as supplied by publisher]
PMCID:
PMC90956
Free PMC Article

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