Arabidopsis NPH1: a flavoprotein with the properti...[Science. 1998]

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1: Science. 1998 Nov 27;282(5394):1698-701. Links

Arabidopsis NPH1: a flavoprotein with the properties of a photoreceptor for phototropism.

Christie JM, Reymond P, Powell GK, Bernasconi P, Raibekas AA, Liscum E, Briggs WR.

Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA.

The NPH1 gene of Arabidopsis thaliana encodes a 120-kilodalton serine-threonine protein kinase hypothesized to function as a photoreceptor for phototropism. When expressed in insect cells, the NPH1 protein is phosphorylated in response to blue light irradiation. The biochemical and photochemical properties of the photosensitive protein reflect those of the native protein in microsomal membranes. Recombinant NPH1 noncovalently binds flavin mononucleotide, a likely chromophore for light-dependent autophosphorylation. The fluorescence excitation spectrum of the recombinant protein is similar to the action spectrum for phototropism, consistent with the conclusion that NPH1 is an autophosphorylating flavoprotein photoreceptor mediating phototropic responses in higher plants.

PMID: 9831559 [PubMed - indexed for MEDLINE]

Phototropin LOV domains exhibit distinct roles in regulating photoreceptor function. [Plant J. 2002]
LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. [Proc Natl Acad Sci U S A. 1999]
Arabidopsis nph1 and npl1: blue light receptors that mediate both phototropism and chloroplast relocation. [Proc Natl Acad Sci U S A. 2001]
Mutations in the NPH1 locus of Arabidopsis disrupt the perception of phototropic stimuli. [Plant Cell. 1995]
Photochemical properties of the flavin mononucleotide-binding domains of the phototropins from Arabidopsis, rice, and Chlamydomonas reinhardtii. [Plant Physiol. 2002]
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Arabidopsis NPH1: a flavoprotein with the properties of a photoreceptor for phototropism.

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