EMBO J. 1998 Nov 16;17(22):6739-46.

Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.

Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC.

Source

The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.

Abstract

Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of PVY and the 2b protein of CMV. The HCPro acts by blocking the maintenance of PTGS in tissues where silencing had already been set, whereas the 2b protein prevents initiation of gene silencing at the growing points of the plants. Combined with previous findings that viruses are both activators and targets of PTGS, these data provide compelling evidence that PTGS represents a natural mechanism for plant protection against viruses.

PMID:: 9822616; [PubMed - indexed for MEDLINE]
PMCID:: PMC1171019

Free PMC Article

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing. [Plant Cell. 1999]
RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing.
Jones L, Hamilton AJ, Voinnet O, Thomas CL, Maule AJ, Baulcombe DC. Plant Cell. 1999 Dec; 11(12):2291-301.
Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. [EMBO J. 2000]
Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus.
Lucy AP, Guo HS, Li WX, Ding SW. EMBO J. 2000 Apr 3; 19(7):1672-80.
Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. [Plant J. 2001]
Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector.
Thomas CL, Jones L, Baulcombe DC, Maule AJ. Plant J. 2001 Feb; 25(4):417-25.
Cucumber mosaic virus infection transiently breaks dsRNA-induced transgenic immunity to Potato virus Y in tobacco. [Mol Plant Microbe Interact. 2003]
Cucumber mosaic virus infection transiently breaks dsRNA-induced transgenic immunity to Potato virus Y in tobacco.
Mitter N, Sulistyowati E, Dietzgen RG. Mol Plant Microbe Interact. 2003 Oct; 16(10):936-44.
Satellite panicum mosaic virus capsid protein elicits symptoms on a nonhost plant and interferes with a suppressor of virus-induced gene silencing. [Mol Plant Microbe Interact. 2004]
Satellite panicum mosaic virus capsid protein elicits symptoms on a nonhost plant and interferes with a suppressor of virus-induced gene silencing.
Qiu W, Scholthof KB. Mol Plant Microbe Interact. 2004 Mar; 17(3):263-71.

See reviews... See all...

Cited by over 100 PubMed Central articles

A MicroRNA Superfamily Regulates Nucleotide Binding Site-Leucine-Rich Repeats and Other mRNAs. [Plant Cell. 2012]
A MicroRNA Superfamily Regulates Nucleotide Binding Site-Leucine-Rich Repeats and Other mRNAs.
Shivaprasad PV, Chen HM, Patel K, Bond DM, Santos BA, Baulcombe DC. Plant Cell. 2012 Mar; 24(3):859-74. Epub 2012 Mar 9.
Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco. [Sci Rep. 2011]
Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco.
Ziebell H, Murphy AM, Groen SC, Tungadi T, Westwood JH, Lewsey MG, Moulin M, Kleczkowski A, Smith AG, Stevens M, et al. Sci Rep. 2011; 1:187. Epub 2011 Dec 9.
Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein. [Int J Mol Sci. 2011]
Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein.
Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Int J Mol Sci. 2011; 12(8):4975-90. Epub 2011 Aug 5.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Viral pathogenicity determinants are suppressors of transgene silencing in Nicot...
Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.
EMBO J. 1998 Nov 16 ;17(22):6739-46.

PubMed
A novel context for the 'MutT' module, a guardian of cell integrity, in a diphos...
A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase.
EMBO J. 1998 Nov 16 ;17(22):6599-607.

PubMed
A novel complex of membrane proteins required for formation of a spherical nucle...
A novel complex of membrane proteins required for formation of a spherical nucleus.
EMBO J. 1998 Nov 16 ;17(22):6449-64.

PubMed
Side-effects of high pressure irrigation.
Side-effects of high pressure irrigation.
Surg Gynecol Obstet. 1976 Nov ;143(5):775-8.

PubMed
Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate de...
Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate dehydrogenase via structure-based drug design.
J Med Chem. 1998 Nov 19 ;41(24):4790-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: