Abstract
Capillary zone electrophoresis (CZE) in the presence of ethanolamine was used in a micropreparative mode. Sample volumes up to 1 microL could be loaded onto a 100 microns diameter capillary without loss in resolution. Coupled to narrow-bore reversed-phase high-performance liquid chromatography, ethanolamine-CZE allowed the collection of sufficient amounts of pure peptidic material to perform amino acid sequence analysis.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Amino Acid Sequence
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Chromatography, High Pressure Liquid
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Electrophoresis, Capillary / methods*
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Ethanolamine*
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Indicators and Reagents
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Molecular Sequence Data
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Peptide Fragments / chemistry
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Peptide Fragments / isolation & purification
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Proteins / isolation & purification
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Proteins / metabolism
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Sequence Analysis
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Trypsin / metabolism
Substances
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Indicators and Reagents
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Peptide Fragments
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Proteins
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Ethanolamine
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Trypsin