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Zhongguo Yao Li Xue Bao. 1996 Jul;17(4):348-50.

Protective effects of tetrandrine on CCl4-injured hepatocytes.

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  • 1Department of Pharmacology, Third Military Medical College, Chongqing, China.

Abstract

AIM:

To study the protective effects of tetrandrine (Tet) on CCl4-injured hepatocytes.

METHODS:

The cultured rat liver cells were poisoned by CCl4 (10 mmol.L-1). The membrane fluidity was detected by 1,6-diphenyl-1,3,5-hexatriene (DPH), a lipid probe. The Ca2+ concentration was assayed with Fura 2-AM, a sensitive calcium indicator.

RESULTS:

Tet (1-1000 nmol.L-1) increased viability of liver cell (from 71% to 72%-89%), reduced lactate dehydrogenase (LDH) release, and malondialdehyde (MDA) formation. Tet prevented the heightening of the intracellular Ca2+ concentration and the attenuation of the membrane fluidity of liver cells (P < 0.05).

CONCLUSION:

Tet had a protective effect on CCl4-injured hepatocytes by inhibiting the lipid peroxidation, improving the membrane fluidity, and lessening the Ca2+ concentration.

PMID:
9812721
[PubMed - indexed for MEDLINE]
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