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    Plant Physiol. 1998 Nov;118(3):935-43.

    Biosynthesis of lipoic acid in Arabidopsis: cloning and characterization of the cDNA for lipoic acid synthase.

    Yasuno R, Wada H.

    Source

    Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan.

    Abstract

    Lipoic acid is a coenzyme that is essential for the activity of enzyme complexes such as those of pyruvate dehydrogenase and glycine decarboxylase. We report here the isolation and characterization of LIP1 cDNA for lipoic acid synthase of Arabidopsis. The Arabidopsis LIP1 cDNA was isolated using an expressed sequence tag homologous to the lipoic acid synthase of Escherichia coli. This cDNA was shown to code for Arabidopsis lipoic acid synthase by its ability to complement a lipA mutant of E. coli defective in lipoic acid synthase. DNA-sequence analysis of the LIP1 cDNA revealed an open reading frame predicting a protein of 374 amino acids. Comparisons of the deduced amino acid sequence with those of E. coli and yeast lipoic acid synthase homologs showed a high degree of sequence similarity and the presence of a leader sequence presumably required for import into the mitochondria. Southern-hybridization analysis suggested that LIP1 is a single-copy gene in Arabidopsis. Western analysis with an antibody against lipoic acid synthase demonstrated that this enzyme is located in the mitochondrial compartment in Arabidopsis cells as a 43-kD polypeptide.

    PMID:
    9808738
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC34804
    Free PMC Article

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      Cited by 8 PubMed Central articles

      • Acyl-lipid metabolism. [Arabidopsis Book. 2013]
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        Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, Debono A, Durrett TP, et al. Arabidopsis Book. 2013; 11:e0161. Epub 2013 Jan 29.
      • Review Lipoic acid metabolism of Plasmodium--a suitable drug target. [Curr Pharm Des. 2012]
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        Storm J, Müller S. Curr Pharm Des. 2012; 18(24):3480-9.
      • Acyl-lipid metabolism. [Arabidopsis Book. 2010]
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        Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, Debono A, Durrett TP, et al. Arabidopsis Book. 2010; 8:e0133. Epub 2010 Jun 11.
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