N Engl J Med. 1976 Nov 18;295(21):1165-7.

Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybridization.

Abstract

The technic of DNA-DNA hybridization was used for prenatal diagnosis of a pregnancy at risk for homozygous alpha-thalassemia. Fibroblasts were cultured from amniotic fluid, and the number of alpha-globin genes in the DNA was quantified by hybridization with radioactive DNA complementary to alpha-globin mRNA sequences. As compared to control studies of DNA from patients with alpha-thalassemia syndromes and from unaffected subjects, the results indicated that the fetus had alpha-thalassemia-1. The diagnosis was confirmed by umbilical-cord blood studies.

PMID:: 980019; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Supplemental Content

Save items

Related citations in PubMed

Prenatal diagnosis of hemoglobin H disease. [J Pediatr. 1978]
Prenatal diagnosis of hemoglobin H disease.
Koenig HM, Vedvick TS, Dozy AM, Golbus MS, Kan YW. J Pediatr. 1978 Feb; 92(2):278-81.
Diagnosis of homozygous alpha-thalassemia in cultured amniotic-fluid fibroblasts. [N Engl J Med. 1978]
Diagnosis of homozygous alpha-thalassemia in cultured amniotic-fluid fibroblasts.
Wong V, Ma HK, Todd D, Golbus MS, Dozy AM, Kan YW. N Engl J Med. 1978 Mar 23; 298(12):669-70.
Application of endonuclease mapping to the analysis and prenatal diagnosis of thalassemias caused by globin-gene deletion. [N Engl J Med. 1978]
Application of endonuclease mapping to the analysis and prenatal diagnosis of thalassemias caused by globin-gene deletion.
Orkin SH, Alter BP, Altay C, Mahoney MJ, Lazarus H, Hobbins JC, Nathan DG. N Engl J Med. 1978 Jul 27; 299(4):166-72.
Review Prenatal diagnosis of thalassemias and hemoglobinopathies. [Ann Clin Lab Sci. 1980]
Review Prenatal diagnosis of thalassemias and hemoglobinopathies.
Seale TW, Rennert OM. Ann Clin Lab Sci. 1980 Sep-Oct; 10(5):383-94.
Review Carrier screening for thalassemia and hemoglobinopathies in Canada. [J Obstet Gynaecol Can. 2008]
Review Carrier screening for thalassemia and hemoglobinopathies in Canada.
Langlois S, Ford JC, Chitayat D, Désilets VA, Farrell SA, Geraghty M, Nelson T, Nikkel SM, Shugar A, Skidmore D, et al. J Obstet Gynaecol Can. 2008 Oct; 30(10):950-71.

See reviews... See all...

Cited by 12 PubMed Central articles

Fetal gene therapy of alpha-thalassemia in a mouse model. [Proc Natl Acad Sci U S A. 2007]
Fetal gene therapy of alpha-thalassemia in a mouse model.
Han XD, Lin C, Chang J, Sadelain M, Kan YW. Proc Natl Acad Sci U S A. 2007 May 22; 104(21):9007-11. Epub 2007 May 11.
delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees. [Proc Natl Acad Sci U S A. 1980]
delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees.
Rizzetto M, Hoyer B, Canese MG, Shih JW, Purcell RH, Gerin JL. Proc Natl Acad Sci U S A. 1980 Oct; 77(10):6124-8.
Review The thalassemias: molecular mechanisms of human genetic disease. [Am J Hum Genet. 1983]
Review The thalassemias: molecular mechanisms of human genetic disease.
Spritz RA, Forget BG. Am J Hum Genet. 1983 May; 35(3):333-61.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybri...
Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybridization.
N Engl J Med. 1976 Nov 18 ;295(21):1165-7.

PubMed
Retention in treatment of heroin users in Italy: the role of treatment type and ...
Retention in treatment of heroin users in Italy: the role of treatment type and of methadone maintenance dosage.
Drug Alcohol Depend. 1998 Oct 1 ;52(2):167-71.

PubMed
[Volume replacement in critically ill intensive-care patients. No classic review...
[Volume replacement in critically ill intensive-care patients. No classic review].
Anaesthesist. 1998 Sep ;47(9):778-85.

PubMed
Neurocysticercosis.
Neurocysticercosis.
Postgrad Med J. 1998 Jun ;74(872):321-6.

PubMed
YAC rescue of downless locus mutations in mice.
YAC rescue of downless locus mutations in mice.
Mamm Genome. 1998 Nov ;9(11):863-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: