Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Nat Biotechnol. 1998 Oct;16(10):946-50.

Identification of a calcium channel modulator using a high throughput yeast two-hybrid screen.

Author information

  • 1Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ 08543, USA.

Erratum in

  • Nat Biotechnol 1998 Nov;16(11):1074.


The interaction of the N-type calcium channel beta3 subunit with the alpha1B subunit alters the activation/inactivation kinetics and the maximal conductance of the channel. The defined protein-protein interaction of the human alpha1B and beta3 subunits provides a target for small-molecule modulation of N-type channel activity. We describe a high throughput screen based on a counterselection yeast two-hybrid assay, which was used to identify small molecules that disrupt alpha1B-beta3 subunit interactions and inhibit N-type calcium channel activity. These small molecules may be a new class of calcium channel antagonists with therapeutic potential.

Comment in

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk