Trends Biochem Sci. 1998 Sep;23(9):331-6.

Structure-specific DNA cleavage by 5' nucleases.

Source

Celltech Therapeutics, Slough, Berkshire, UK.

Abstract

Many processes, such as DNA replication, recombination and repair, produce branched DNA structures. These bifurcated molecules are trimmed by a group of homologous 5'-3' exonucleases (also known as 5' nucleases) in structure-specific cleavage reactions. X-ray crystallographical and biochemical studies suggest that ssDNA substrates become threaded through the 5'-3' exonucleases, where hydrolysis is effected with the aid of divalent metal cations.

PMID:: 9787638; [PubMed - indexed for MEDLINE]

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Wellcome Trust/United Kingdom

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Structures reported by this article

T5 5'-Exonuclease Mutant K83a

PDB: 1XO1

Source: Enterobacteria phage T5

Method: X-Ray Diffraction

Resolution: 2.5 Å

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