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Int Arch Allergy Immunol. 1998 Sep;117 Suppl 1:28-33.

Heterogeneous expression of tumor necrosis factor-alpha receptors I and II on human peripheral eosinophils.

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  • 1Department of Pediatrics, Toho University School of Medicine, Sakura Hospital, Sakura, Japan.

Abstract

BACKGROUND:

Expression of the tumor necrosis factor-alpha receptor by human eosinophils has not been determined. We examined the surface expression and mRNA for tumor necrosis factor-alpha receptors I and II (TNF-alphaRI and TNF-alphaRII) on peripheral eosinophils.

METHODS:

Eosinophils were obtained from 17 asthma patients and 3 healthy volunteers. Flow cytometry was used to examine receptor expression, and the reverse transcriptase-polymerase chain reaction was used to examine receptor mRNA expression.

RESULTS:

Flow cytometry revealed that 16 out of 20 subjects had eosinophils expressing TNF-alphaRI and 18 subjects had eosinophils expressing TNF-alphaRII. All eosinophils expressed at least one receptor. The mRNA for TNF-alphaRII was detected in all eosinophils examined, but the signals for TNF-alphaRI mRNA were only found in cells expressing this receptor.

CONCLUSIONS:

Human peripheral eosinophils show heterogeneous expression of TNF-alphaRI and TNF-alphaRII.

PMID:
9758893
[PubMed - indexed for MEDLINE]
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