J Histochem Cytochem. 1998 Oct;46(10):1097-102.

Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle.

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Department of Anatomy, Jichi Medical School, Tochigi, Japan.

Abstract

We demonstrate a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to be a versatile reporter system for immunocytochemical labeling of ultrathin cryosections. FNG-labeled molecules in the same ultrathin cryosections can be resolved by two imaging techniques (i.e., fluorescence and electron microscopy). Lactoferrin, a marker protein for the specific granules in human neutrophils, was employed as the target for FNG immunolabeling. The spatial resolution of the fluorescence signal from FNG-labeled specific granules was compatible with that of silver-enhanced gold signal from the same granules in electron microscopy. Our results confirm that FNG can be used as a probe for high-resolution correlation between immunofluorescence and electron microscopy.

PMID:: 9742065; [PubMed - indexed for MEDLINE]

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Lactoferrin

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HD35121/HD/NICHD NIH HHS/United States

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