Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Microbiol. 1998 Oct;37(4):281-8.

Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier Pseudomonas putida.

Author information

  • 1Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany.

Abstract

The heterotrophic nitrifier Pseudomonas putida aerobically oxidized ammonia to hydroxylamine, nitrite, and nitrate. Product formation was accompanied by a small but significant release of NO, whereas N2O evolution could not be detected under the assay conditions employed. The isolate reduced nitrate to nitrite and partially further to NO under anaerobic conditions. Aerobically grown cells utilized gamma-aminobutyrate as a carbon source and as a N-source by ammonification. The physiological experiments, in particular the inhibition pattern by C2H2, indicated that P. putida expressed an ammonia monooxigenase. DNA-hybridization with an amoA gene probe coding for the smaller subunit of the ammonia monooxigenase of Nitrosomonas europaea allowed us to identify, to clone, and to sequence a region with an open reading frame showing distinct sequence similarities to the amoA gene of autotrophic ammonia oxidizers.

PMID:
9732537
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk