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    Genomics. 1998 Jul 15;51(2):299-302.

    Genomic organization and chromosomal localization of the human SGK protein kinase gene.

    Waldegger S, Erdel M, Nagl UO, Barth P, Raber G, Steuer S, Utermann G, Paulmichl M, Lang F.

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    Department of Physiology I, University of Tübingen, Germany. siegfried.waldegger@uni-tuebingen.de

    Abstract

    The SGK protein kinase is a novel member of the serine/threonine protein kinase family. Its corresponding gene belongs to the group of immediate-early genes. SGK transcription is controlled by cell volume alterations in different cell lines. To analyze the genomic structure and chromosomal location of the SGK gene, a human P1 clone was isolated by screening a human genomic library with a SGK cDNA probe. This clone was confirmed to encode the authentic SGK gene by the detection of exon-intron structures and the correspondence between the nucleotide sequences of exons and human cDNA. Using this P1 clone as a probe for fluorescence in situ hybridization, a single chromosomal locus for SGK was assigned to band 6q23, a region frequently affected by deletion in various human neoplasms.

    PMID:
    9722955
    [PubMed - indexed for MEDLINE]

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