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J Cell Biol. 1998 Aug 24;142(4):1053-62.

The Ras target AF-6 is a substrate of the fam deubiquitinating enzyme.

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  • 1Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-0101, Japan.

Abstract

The Ras target AF-6 has been shown to serve as one of the peripheral components of cell-cell adhesions, and is thought to participate in cell-cell adhesion regulation downstream of Ras. We here purified an AF-6-interacting protein with a molecular mass of approximately 220 kD (p220) to investigate the function of AF-6 at cell-cell adhesions. The peptide sequences of p220 were identical to the amino acid sequences of mouse Fam. Fam is homologous to a deubiquitinating enzyme in Drosophila, the product of the fat facets gene. Recent genetic analyses indicate that the deubiquitinating activity of the fat facets product plays a critical role in controlling the cell fate. We found that Fam accumulated at the cell-cell contact sites of MDCKII cells, but not at free ends of plasma membranes. Fam was partially colocalized with AF-6 and interacted with AF-6 in vivo and in vitro. We also showed that AF-6 was ubiquitinated in intact cells, and that Fam prevented the ubiquitination of AF-6.

PMID:
9722616
[PubMed - indexed for MEDLINE]
PMCID:
PMC2132865
Free PMC Article
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