Trends Cell Biol. 1998 Jul;8(7):288-92.

Digital imaging microscopy of living cells.

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Dept of Biomedical Sciences, University of Padova, Italy. rizzuto@civ.bio.unipd.it

Abstract

Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.

PMID:: 9714601; [PubMed - indexed for MEDLINE]

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