Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 1998 Aug 21;281(5380):1194-7.

Direct allelic variation scanning of the yeast genome.

Author information

  • 1Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA. winzeler@cmgm.stanford.edu

Abstract

As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).

Comment in

PMID:
9712584
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire
    Loading ...
    Write to the Help Desk