Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 1998 Sep;18(9):5355-63.

A human RNA polymerase II complex containing factors that modify chromatin structure.

Author information

  • 1Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.

Abstract

We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.

PMID:
9710619
[PubMed - indexed for MEDLINE]
PMCID:
PMC109120
Free PMC Article

Images from this publication.See all images (5)Free text

FIG. 1
FIG. 2
FIG. 3
FIG. 4
FIG. 5
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk