SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors.
Laherty CD,
Billin AN,
Lavinsky RM,
Yochum GS,
Bush AC,
Sun JM,
Mullen TM,
Davie JR,
Rose DW,
Glass CK,
Rosenfeld MG,
Ayer DE,
Eisenman RN.
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30, SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
PMID: 9702189 [PubMed - indexed for MEDLINE]