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Cell. 1998 Jul 24;94(2):205-16.

PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization.

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  • 1Vollum Institute, The Oregon Health Sciences University, Portland 97201, USA.

Abstract

We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS-1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell-free assays indicate TGN localization of furin is directed by a PACS-1-mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

PMID:
9695949
[PubMed - indexed for MEDLINE]
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