Nature. 1998 Jul 23;394(6691):337-43.

The structural basis of the activation of Ras by Sos.

Boriack-Sjodin PA, Margarit SM, Bar-Sagi D, Kuriyan J.

Source

Laboratory of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA.

Abstract

The crystal structure of human H-Ras complexed with the Ras guanine-nucleotide-exchange-factor region of the Son of sevenless (Sos) protein has been determined at 2.8 A resolution. The normally tight interaction of nucleotides with Ras is disrupted by Sos in two ways. First, the insertion into Ras of an alpha-helix from Sos results in the displacement of the Switch 1 region of Ras, opening up the nucleotide-binding site. Second, side chains presented by this helix and by a distorted conformation of the Switch 2 region of Ras alter the chemical environment of the binding site for the phosphate groups of the nucleotide and the associated magnesium ion, so that their binding is no longer favoured. Sos does not impede the binding sites for the base and the ribose of GTP or GDP, so the Ras-Sos complex adopts a structure that allows nucleotide release and rebinding.

Comment in

Ras signalling. Caught in the act of the switch-on. [Nature. 1998]
Ras signalling. Caught in the act of the switch-on.Wittinghofer F. Nature. 1998 Jul 23; 394(6691):317, 319-20.

PMID:: 9690470; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Complex Of Human H-Ras With Human Sos-1

PDB: 1BKD

Source: Homo sapiens

Method: X-Ray Diffraction

Resolution: 2.8 Å

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